1/28/2024 0 Comments Seaturtle sketch![]() These animals do not have sex chromosomes and instead, sex is determined by the temperature during egg incubation. One potential group of concern is reptiles with temperature dependent sex determination ( Janzen, 1994 Mitchell and Janzen, 2010 Blechschmidt et al., 2020). Future research into sex-specific differentially methylated regions in hatchlings and juveniles should be based on genome-wide DNA methylation data from a wider age range, which includes hatchlings. Epigenetic biomarkers are a promising tool for wildlife research because they can be minimally invasive and high throughput. This suggests the sex differences distinguishable by the assay were adult specific, reflecting the training data on which the sex-specific regions were identified, and likely linked to late-stage ontogenetic changes associated with sexual maturity. However, the sex of younger turtles could not be accurately identified. We used reduced representation bisulfite sequencing DNA methylation data from adult green sea turtle ( Chelonia mydas) skin biopsies to identify 16 genomic regions exhibiting differential methylation between males and females (adjusted p-value 17 years. In this study, we aimed to develop a rapid, minimally invasive, and inexpensive method to identify the sex of marine turtles. Neither have epigenetic biomarkers for sex been developed into rapid assays suited to research on wildlife. Changes in DNA methylation, an epigenetic modification, have been characterized as part of sex differentiation pathways in some species with environmentally determined sex, but so far not in marine turtles. ![]() However, available methods are typically lethal, highly invasive, or difficult to conduct at a large scale. Assessment of this risk at the population level requires the identification of sex in hatchlings and juveniles. Global warming may increase the extinction risk by skewing hatchling sex ratios. In marine turtles, the sex of hatchlings is determined by their egg incubation temperature. 6Marine Science Program, Department of Biodiversity, Conservation and Attractions, Kensington, WA, Australia.5Commonwealth Scientific and Industrial Research Organisation Commonwealth Scientific and Industrial Research Organisation (CSIRO), Crawley, WA, Australia.4Kélonia l’observatoire des tortues marines, Saint-Leu, La Réunion, France.3Centre d’Etude et de Découverte des Tortues Marines (CEDTM), Piton Saint-Leu, La Réunion, France.2Cayman Turtle Conservation and Education Centre, Grand Cayman, Cayman Islands.1CSIRO Environomics Future Science Platform, Indian Oceans Marine Research Centre, Commonwealth Scientific and Industrial Research Organisation Commonwealth Scientific and Industrial Research Organisation (CSIRO), Crawley, WA, Australia. ![]() Benjamin Mayne 1*, Walter Mustin 2, Vandanaa Baboolal 2, Francesca Casella 2, Katia Ballorain 3, Mathieu Barret 4, Mathew A.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |